Feces Container, The 30mL screw cap container with a spoon is ideal for the collection and transport of fecal specimens. Available in transparent polystyrene (PS) or opaque polypropylene (PP), these stoolcontainers come completely assembled and are ready to use.
The FastDNA-96™ Fecal DNA Kit isolates bacterial, protist, or host DNA from up to 80 mg of wet or dry stool, and even from caecum samples, through a purification process that eliminates PCR inhibitors. Fecal microbes, fungi, parasites, and other stool organisms are first lysed in approximately 60 seconds with the FastPrep-96™ Instrument.Up to 80 mg of wet or dry feces are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack (96-deep well plate) containing 0.5 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of eukaryotic and prokaryotic organisms present in the mammalian stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.), while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, Feces Container and spin-column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes humic acids, polyphenols that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, methylation, and PCR (A260/A280 ratios = 1.8). Yield is typically 5 µg of total DNA eluted in 50-100 µL of elution solution. This procedure can be performed in minutes, Feces Container and there is no need for organic denaturants or proteinases.
Collecting a Stool Specimen
Unlike most other lab tests, the stool is sometimes collected by the child's family at home, not by a health care professional. Here are some tips for collecting a stool specimen:
Collecting stools can be messy, so be sure to wear latex gloves and wash your hands and your child's hands well afterward.
Many kids with diarrhea, especially young children, can't always let a parent know in advance when a bowel movement is coming. Sometimes a hat-shaped plastic lid is used to collect the stool specimen. This catching device can be quickly placed over the toilet bowl or your child's rear end to collect the specimen. Using a catching device can prevent contamination of the stool by water and dirt. If urine contaminates the stool sample, it will be necessary to take another sample. Also, if you're unable to catch the stool sample before it touches the inside of the toilet, the sample will need to be repeated. Fishing a bowel movement out of the toilet does not provide a clean specimen for the laboratory to analyze.
Another way to collect a stool sample is to loosely place plastic wrap across the rim of the toilet, under the seat. Then place the stool sample in a clean, sealable container before taking it to the laboratory. Plastic wrap can also be used to line the diaper of an infant or toddler who is not yet using the toilet.